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KMID : 1101420190510040444
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Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 4 p.444 ~ p.452
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High Prevalence and Genotypic Characterization of Metallo-?-Lactamase (MBL)-Producing Acinetobacter spp. Isolates Disseminated in a Korean Hospita
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Yum Jong-Hwa
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Abstract
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This study investigated the improved lipid metabolism effect of 3T3-L1 cells induced by adipocytes using the dichloromethane (DCM) fraction in the organic solvent extract of Wassong (Orostachys japonicus). To confirm the cell cytotoxicity, each of 6 fractions of organic solvent extracts (EtOH, Hexane, DCM, EtOAc, BuOH, and H2O) was examined using MTS assay. As a result, it was confirmed that the DCM extract was stable over the whole range of concentrations, and a DCM fraction was used to confirm the improved lipid metabolism effect. Lipid excretion was measured to confirm the change of lipid metabolism. 3T3-L1 cells induced by adipocytes were treated with DCM extract and stained with oil-red O to evaluate lipid accumulation. As a result, it was confirmed that the lipid efflux was significantly improved. In order to confirm the mechanism of lipid efflux, the mRNA expressions of ABCA1 and ABCG1, which are lipid transport proteins, were confirmed by real-time PCR. Therefore, the present study confirmed that the DCM extract from Orostachys japonicus has the effect of improving the lipid metabolism on 3T3-L1 adipocytes. In addition, the results of this study will be used as the basis for the development of functional foods using Orostachys japonicus and also for conducting research on the detailed mechanisms.
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KEYWORD
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ATP-binding cassette sub-family- A member 1 (ABCA1), ATP-binding cassette sub-family- G member 1 (ABCG1), Lipid metabolism, Orostachys japonicus, 3T3-L1
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